Poly-lactic acid (PLA) is currently the most widely used material, with low warpage, excellent melt fluidity and adhesion, no unpleasant odor when printing, excellent mechanical properties, which is favored by the industry as a biodegradable material. However, due to the excessive brittleness of PLA, its further application is limited. Chitosan is a biomass polysaccharide extracted mainly from the shells of shrimp and crab wastes. It is rich in reserves in nature and is the second largest biomass polysaccharide compared to cellulose. It has characteristics such as biocompatibility, biodegradability, bacteriostasis, adsorption performance, and non-toxicity. To achieve the adsorption and antibacterial effects of 3D printing materials, it is necessary to increase the content of chitosan to expose more chitosan on the surface of wires and products. Herein, we made 3D-printed PLA/Chitosan and used in the gynaecological BeWo cell line. To investigate the treatment effect in inflammation of the female reproductive system, different matching types of 3D-printed PLA/Chitosan and printing parameters were tried till the optimal one was chosen to run the proteomic analysis. A mouse model of gynecological inflammation was established and the comparison of those two cell lines with and without 3D-printed PLA/Chitosan in treatment were analysed. The differential expressed proteins were identified by mass spectroscopy. Our work established a connection between the 3D printing process and the biological materials, which provided assistance in developing PLA/Chitosan-3D printing based applications and the potential disease marker can be found to guide the treatment. Of gynecological diseases.